http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2021102239-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_7ef01e33058cf740cf2197991be97af1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d768a8052fb947a70bfc3ee23261a2f3 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-689 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-686 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-686 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-689 |
| filingDate | 2018-06-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d8020880b2677b04b4c7c668052da592 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b7620f06aff48b2af074a4d280a57c10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c685bc6eff42cdbb88a88004d5c52735 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f1e82e7485973ca5374d08277bfb2500 |
| publicationDate | 2021-04-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | US-2021102239-A1 |
| titleOfInvention | Method for determining bacterial number in specimen |
| abstract | A method for enabling rapid and accurate determination of the number of bacterial cells in a specimen using a PCR method includes the following steps: (1) a first PCR step of carrying out a PCR method using a nucleic acid derived from a specimen as a template and a universal primer pair for amplifying a bacterial 16S rRNA gene to obtain a first amplification product; (2) a second PCR step of carrying out a nested PCR method using a primer pair(s) for amplifying an internal sequence(s) of the sequence of the first amplification product obtained by the first PCR step to obtain a second amplification product; and (3) a bacterial number determination step of obtaining the number of bacterial cells in the specimen based on the amount of the second amplification product obtained in the second PCR step and using calibration data. |
| priorityDate | 2017-12-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 201.