http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2021102227-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_2e39a288d149ab88e19e5b980e72fd1e |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12R2001-785 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N1-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-80 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0071 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P7-6427 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1029 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P7-6463 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P7-6431 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P7-6436 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N1-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-80 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P7-64 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-80 |
| filingDate | 2020-12-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_afe200dbec18faeaf5a25cfd6b8cd899 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8e4afc6b44ae144e13324dcb70773b6a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3568a2fdd43d46b79c79cb19b413d2b3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_83819d2f4a451e0ad5984f5272621832 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_27ab743710c537c29710b8693b7ce95f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1e93fd91ceae12ee0d5d09119db1ea1d |
| publicationDate | 2021-04-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | US-2021102227-A1 |
| titleOfInvention | Construction method of mucor circinelloides cell factory for producing dihomo-gamma-linolenic acid and fermentation technology |
| abstract | The present disclosure relates to a construction method of a Mucor circinelloides cell factory for producing dihomo-γ-linolenic acid and a fermentation technology, belonging to the field of genetic engineering. In the present disclosure, γ-linolenic acid elongase gene glelo is obtained from Mortierella alpine by cloning, the gene is ligated to an integrative plasmid pMAT1552, and transformed into a Mucor circinelloides defective strain Mu402, and the gene glelo is integrated into Mucor circinelloides genome through homologous recombination, to obtain the recombinant strain Mc-glelo, and finally, the expression of the gene glelo in Mucor circinelloides is realized. The lipid content in the recombinant strain Mc-glelo is not obviously different from that in the control strain Mc1552, however, the lipid composition changes greatly, and dihomo-γ-linolenic acid appears in the lipids of the recombinant strain Mc-glelo, and the content thereof reaches 5.7% of the total fatty acids. Under optimized fermentation conditions and in the presence of precursor fatty acid, the DGLA content reaches 7.6%. The new recombinant strain was deposited in China General Microbiological Culture Collection Center on Jun. 20, 2018, with the address of No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing. The accession number given to the biological material by the collection center is CGMCC No. 15887, and the suggested taxonomic denomination is Mucor circinelloides-GLELO. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114410482-A |
| priorityDate | 2018-12-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 83.