| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_70f85bbf7d491932e1438b3a1bbd72a8
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_c4f66696a0f55c052efce5e78b6cd73e
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_25ede86d145633f7f35160ec5242d5b7
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_bb287ae977d4c10f2e9fe5afe55ac858
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_bf354906514b85a3f80ef5ea4c575314
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_c345997479c0cac4786465abcb859042 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-38
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-165
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2521-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-998
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-999
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2506-1384 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K35-28
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0667
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0654 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-0775
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K35-28 |
| filingDate |
2019-07-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_966732805d3130fd86a00fdec5fcc770
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5ced2ed182f1a292d964bd50222072de
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c41ff819bf194974ed4ea2891794aa40
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b55d972f0d5820812b906eabc6099281
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_961f5fcdba47a468d7cb403a50c8d100
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_469a3863e97020306e13276b5df3384a |
| publicationDate |
2021-01-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-2021009959-A1 |
| titleOfInvention |
Methods for enhancing osteogenic differentiation using vitamin d |
| abstract |
A method of enhancing osteogenic differentiation using vitamin D treatment is disclosed. The method utilizes the combined effect of vitamin D treatment and flow-induced shear stress in a modified perfusion bioreactor to treat the bone defect. The method comprising the steps of: isolating adipose tissue from a subject by liposuction; separating adipose-derived stem cells from the adipose tissue; pre-treating the separated adipose-derived stem cells for a predefined time 20 to 40 minutes with vitamin D3; seeding the pre-treated stem cells onto one or more scaffolds; washing of unattached stem cells from the scaffolds after a predefined time of 20 to 30 minutes; culturing the stem cell seeded scaffold by utilizing a modified perfusion bioreactor to form a tissue-engineered construct, where flow induced shear stress is applied, and implanting the tissue-engineered construct into the subject without a need to obtain autologous bone graft. |
| priorityDate |
2019-07-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |