http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2020385712-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_80787665b837ed3eb503bbcd27c0043a |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1096 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6837 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6837 |
| filingDate | 2018-11-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_93b41e57622c1d880ab0bb7491575e4a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d5d660e334a08df220be1c2146bde4e5 |
| publicationDate | 2020-12-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | US-2020385712-A1 |
| titleOfInvention | Method for comprehensively analyzing 3' end gene expression of single cell |
| abstract | A method for analyzing gene expression in a cell using a device, the method including: introducing a plurality of cells into microreactors so that a single cell corresponds to each microreactor, capturing mRNA from the single cell on the probe, synthesizing first cDNA by subjecting the captured mRNA to a reverse transcription reaction, to produce a first cDNA library derived from the single cell on the solid supports, washing the solid supports, a step of synthesizing second cDNA from the first cDNA library, performing fragmentation of double-stranded DNA containing the first and second cDNA and addition of a tag sequence, removing a component other than an immobilized double-stranded DNA fragment by washing the solid supports with a washing solution, performing amplification of the double-stranded DNA fragment, and performing, for the amplified sequence, analysis of gene expression in each single cell, using the cell identification sequence and the molecule identification sequence. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113322523-A |
| priorityDate | 2017-12-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
| Predicate | Subject |
|---|---|
| isDiscussedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/substance/SID226399539 http://rdf.ncbi.nlm.nih.gov/pubchem/compound/CID6235 |
Total number of triples: 17.