http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2020378969-A1
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_0cde7745e014bc36f84bbd7046ca93fd |
classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N1-30 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-5428 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-54 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-495 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-4704 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-4703 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-715 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-70517 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-7051 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-56972 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-505 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-533 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N1-30 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-569 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N1-30 |
filingDate | 2018-06-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_edac8ca6fbd4ad9a7be96bb4e1f9f9a5 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f9abb7fd1816f15c60f085bfbb7c25a2 |
publicationDate | 2020-12-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | US-2020378969-A1 |
titleOfInvention | Kit for detecting subset of human regulatory t cell, and detection method thereof |
abstract | The present invention relates to a kit for detecting a subset of a human regulatory T cell, and a detection method thereof, and belongs to the technical field of cell subset detection. The present invention provides a kit which includes the following components: a blood dilution solution, a mononuclear cell isolation solution, a cell culture solution, a lymphocyte activation liquid, a dead-cell-removing dye, an FcR blocking agent, fluorescence-labeled antibodies against human cell-surface markers, fluorescence-labeled antibodies against human intracellular molecules, a PBS buffer, lipopolysaccharide, a washing buffer, a cell staining buffer, a cell fixing solution, and a membrane-permeable wash buffer. At the time of detection, firstly mononuclear cells are isolated from whole blood, secondly human peripheral blood mononuclear cells are stimulated, and finally the subsets of the regulatory T cells can be detected just by fluorescent antibody staining. Use of the kit and detection method of the present invention can identify 2-6 subsets of regulatory T cells quickly and easily. |
priorityDate | 2018-04-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 57.