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filingDate 2018-06-13-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_edac8ca6fbd4ad9a7be96bb4e1f9f9a5
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publicationDate 2020-12-03-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber US-2020378969-A1
titleOfInvention Kit for detecting subset of human regulatory t cell, and detection method thereof
abstract The present invention relates to a kit for detecting a subset of a human regulatory T cell, and a detection method thereof, and belongs to the technical field of cell subset detection. The present invention provides a kit which includes the following components: a blood dilution solution, a mononuclear cell isolation solution, a cell culture solution, a lymphocyte activation liquid, a dead-cell-removing dye, an FcR blocking agent, fluorescence-labeled antibodies against human cell-surface markers, fluorescence-labeled antibodies against human intracellular molecules, a PBS buffer, lipopolysaccharide, a washing buffer, a cell staining buffer, a cell fixing solution, and a membrane-permeable wash buffer. At the time of detection, firstly mononuclear cells are isolated from whole blood, secondly human peripheral blood mononuclear cells are stimulated, and finally the subsets of the regulatory T cells can be detected just by fluorescent antibody staining. Use of the kit and detection method of the present invention can identify 2-6 subsets of regulatory T cells quickly and easily.
priorityDate 2018-04-13-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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