Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d21cbff64022f95c237c0bd3ec8656ca |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-30 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2506-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2533-52 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-15 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2506-45 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-067 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0676 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0607 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0613 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-074 |
filingDate |
2020-06-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f48122d5409352456b3a66bf6c1b47b6 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bee08eeb4587d48f83c12ef4aefc61ce |
publicationDate |
2020-12-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
US-2020377853-A1 |
titleOfInvention |
Culture conditions to prolong/enhanced endodermal cell type for cell transplantation |
abstract |
The present invention includes a method of preparing differentiated endodermal cells comprising growing human pluripotent stem cells (hPSC) under serum free conditions for 3 days on a layer of fibronectin or a gelatinous protein layer in the presence of high activin/TGF-β with a GSK3 inhibitor and a PI3K/mTORC inhibitor in a basal differentiation medium; adding a knock out serum replacement to the media starting on day 4 through day 7; and harvesting the endodermal cells grown therein. |
priorityDate |
2019-06-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |