http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2020355685-A1

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publicationDate 2020-11-12-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber US-2020355685-A1
titleOfInvention Reagent set for detecting interactions between biomolecules and their regulatory factors, and applications
abstract The present invention discloses a reagent set for detecting interactions between biomolecules and their regulatory factors and applications. One reagent set disclosed in the present invention consists of three reagents named A, B and C; the reagent A is formed by connecting a biomolecule R and a biomolecule X; the reagent B contains a biomolecule L; there is an interaction between the biomolecule R and the biomolecule L, and a phase transition occurs when the biomolecule R and the biomolecule L interact; the reagent C is formed by connecting a reporter group JIA with a biomolecule named X L . Another reagent set disclosed in the present invention consists of four reagents named A, B, E and D; the reagent E is a polymer formed by E monomers, and the E monomer is obtained by connecting a monomer mc, a reporter group JIA, and a biomolecule Y C , two or more mc monomers can form a polymer; the reagent D is formed by connecting a modified protein X L and a biomolecule Y D ; there is an interaction between the biomolecule Y C and the biomolecule Y D . The present invention can be used to detect the intracellular and extracellular protein interaction or even the weak interaction and has the characteristics of high visibility, simple operation, low cost, high sensitivity and wide applicability.
priorityDate 2017-11-06-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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