| abstract |
An object of the present invention is to provide a method for preparing ambrein, which can easily and efficiently obtain the ambrein. n The object can be solved by a mutated tetraprenyl-β-curcumene cyclase whereinn (1) a 4th amino acid residue of a DXDD motif, aspartic acid, is substituted with an amino acid other than aspartic acid, and (2) an amino acid adjacent to the N-terminus of a (A/S/G)RX(H/N)XXP motif is substituted with an amino acid other than tyrosine, or a 4th amino acid of the GXGX(G/A/P) motif is substituted with an amino acid other than leucine. |