Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_cb351a5a4e03ee3773bd0df5dbae9ac3 |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-81 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-66 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A23C9-1206 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-2471 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A23L33-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y302-01023 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-38 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A23L33-21 |
filingDate |
2020-07-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7098a70443b0acff84bbd938e22a7fc9 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2c097723ad11c2b34c14aba8e1f6387e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_389ada4a69cd74e5fee0b70919486133 |
publicationDate |
2020-11-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
US-2020347372-A1 |
titleOfInvention |
Beta-galactosidase Mutant, and Preparation Method and Application Thereof |
abstract |
The present invention discloses a β-galactosidase mutant, and a preparation method and application thereof, belonging to the fields of gene engineering and enzyme engineering. Amino acids of specific sites in the β-galactosidase are mutated, the β-galactosidase is transferred into a recombinant bacterium, and enzymatic transformation is performed under optimized conditions, so that the yield of galactooligosaccharide produced by the mutant reaches 59.8%, which is increased by about 70% as compared with that of wild enzyme, thereby implementing the increase of the galactooligosaccharide yield. The present invention has very high industrialized application value. |
priorityDate |
2018-01-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |