| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_8e75810cb7d022e16fc3e012a68ae090 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-178 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-68
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-682
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6844
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-689 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-682
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6844
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-689 |
| filingDate |
2018-03-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8abc6fc43838cd7148e32ceefa4ec061
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_467b8f955a68414511e6c185dd2fee43
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f108e2b668fc7552f93c379f1d2b702f |
| publicationDate |
2020-10-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-2020325521-A1 |
| titleOfInvention |
Nucleic acid enzyme-mediated signal amplification for biosensing |
| abstract |
Described is an approach that takes advantage of rolling circle amplification (RCA) and an RNA-cleaving DNAzyme (RCD) to achieve massive signal amplification for biosensing via a cross-feedback mechanism. An RCA reaction generates copies of an RCD that triggers a reaction cascade designed to generate additional DNA assemblies for RCA. These cross-feedback actions work autonomously to turn limited molecular recognition events into massive amounts of DNA amplicons that can be conveniently detected. This approach was demonstrated for biosensing of a microRNA sequence and a bacterium. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114292902-A |
| priorityDate |
2017-03-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |