http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2020325484-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_1f04134f9199a9acc3b05e085e207d58 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K67-0333 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2840-20 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-635 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-81 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-63 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-902 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-86 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-63 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-86 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-81 |
| filingDate | 2019-04-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_163178d63277a58cb9e724e9f5634288 |
| publicationDate | 2020-10-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | US-2020325484-A1 |
| titleOfInvention | Enhancing gene expression by linking self-amplifying transcription factor with viral 2A-like peptide |
| abstract | The invention describes a nucleic acid system, named as “2A-transcription amplifier”, for enhancing gene expression by linking a gene of interest (GOI) to a self-amplifying transcription factor with a viral 2A-like peptide. The system comprises an upstream activation sequence (UAS) at upstream promoter region and another sequence encoding a specific transcription factor (TF), a viral 2A-like peptide, and a gene of interest (GOI). The said compositions are operably linked in a way that the initially expressed TF protein binds the UAS region and promotes more TF and GOI co-expression. The viral 2A-like peptide separates the co-expressed TF and GOI protein during protein translation by the mechanism of ribosomal skidding. The system creates a transcription amplification loop that can be employed for enhancing expression of exogenous or endogenous gene of interest (GOI) in eukaryotic cells, tissues or whole organisms. |
| priorityDate | 2019-04-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 497.