http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2020300874-A1

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filingDate 2018-11-02-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e77a7ba2e62026162d0a9cc5abf501c3
publicationDate 2020-09-24-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber US-2020300874-A1
titleOfInvention Method and kit for sample preparation and endotoxin determination
abstract The invention relates to a method for preparation of a sample (10) of a formulation (11) for subsequent endotoxin determination, the formulation (11) suspected of comprising an endotoxin, the formulation (11) preferentially being a pharmaceutical formulation. The method comprises the following steps: application of the sample (10) to an endotoxin-free centrifugation column (2) containing a size exclusion chromatography matrix (5) that has been equilibrated with a suitable equilibration buffer (6) and elution of a flow through (15) of the sample by centrifugation, which flow through (15) can then be used for endotoxin determination. The equilibration buffer (6) is selected according to a subsequently used method of endotoxin determination, the equilibration buffer (6) only containing components not interfering with subsequently used method of endotoxin determination. Furthermore, the invention relates to a kit (20) for preparation of a sample (10).
isCitedBy http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112462015-A
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