| abstract |
This disclosure relates to the field of high scale production of recombinant Adeno-Associated Viruses (AAVs). The inventors have conceived of specific nucleic acid constructs that allow for high scale production of recombinant AAV particles in insect cells. Importantly, these nucleic constructs do not require the production of a heterologous AAP. This disclosure thus relates to a nucleic acid for producing AAV capsids in insect cells, where the nucleic acid includes a first open reading frame encoding the VP1, VP2, and VP3 proteins, and a second open reading frame encoding the Assembly-Activating Protein (AAP). |