http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2020229427-A1

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filingDate 2018-10-12-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_647daf2b47126e69d4f1aab89a1d2d29
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publicationDate 2020-07-23-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber US-2020229427-A1
titleOfInvention Cryopreservation Method and Apparatus
abstract The present invention relates to methods and apparatus for the cryopreservation of biological samples involving a density assisted vitrification wherein a sample of biological material in a cryopreservation agent is cooled from its top surface, causing an ice layer to form thereon. As cooling continues the ice layer grows downwards through the sample to provide a cryoprotectant and biological material rich layer below the ice layer that undergoes vitrification as cooling continues to below the glass transition temperature.
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