| abstract |
The present invention provide the use of Nicotiana benthamiana (N. benthamiana) HIR3s gene and/or Oryza sativa HIR3 gene in producing plants with resistance to virus and the method for making the plants thereof, the method involve: constructing NbHIR3.1, NbHIR3.2 or OsHIR3 into plant binary expression vector pCV1300 respectively, and introduced into Agrobacterium by electric shock, then transgenic plants overexpressing either NbHIR3.1 or NbHIR3.2 gene or tobacco or rice overexpressing HIR3 were produced by infection with Agrobacterium; the nucleotide sequences of NbHIR3.1, NbHIR3.2 and OsHIR3 are shown as SEQ ID NO:1, SEQ ID NO:2 and SEQ ID NO:23 respectively. |