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filingDate 2017-06-22-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 2020-06-04-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber US-2020173960-A1
titleOfInvention Method for Quantifying Monoclonal Antibody Having Antigen or Anti-Antibody Bonded Thereto
abstract The present invention provides a method in which a porous body having a monoclonal antibody to be measured immobilized in pores thereof is brought into contact with nanoparticles having a protease immobilized thereonto in a liquid to perform selective protease digestion of the monoclonal antibody and a peptide fragment obtained by the digestion is detected by liquid chromatography mass spectrometry (LC-MS), wherein the monoclonal antibody is digested with the protease in the presence of an antibody specifically binding to the monoclonal antibody or a target molecule of the monoclonal antibody.
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