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publicationDate 2020-05-14-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber US-2020149096-A1
titleOfInvention Method of eliminating background amplification of nucleic acid targets
abstract An object is to provide a method of eliminating background amplification in detection of rare nucleic acids based on isothermal amplification. The method comprising steps of: preparing a mixture including solution and enzymes, and adding a first and a second template into the mixture, wherein the first template is an amplification template, and the second template is a leak absorption template. Methods to use this approach for multiplexed, ultrasensitive and ultra-specific detection of nucleic acids are presented.
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