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filingDate 2019-12-27-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_92acb69944dae841ab7d98ff8a9f1221
publicationDate 2020-05-07-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber US-2020143539-A1
titleOfInvention Spatial analytical microbial imaging
abstract The present invention relates to microbial sample and standard preparation, staining and labeling, imaging and data acquisition as well as a method and apparatus for distinguishing objects of interest from other objects and background in an optical field. A microbial genomic analysis tool, Spatial Analytical Microbial Imaging (SAMI), provides the spatiotemporal and comparative intracellular ploidy, indicating the relative growth rate of cells in situ. Objects in a 2D or 3D optical field are tagged using fluorescence marker DNA binding to specifically visualize and semi-quantify the targeted objects in the sample, which allows them to be identified characterized and counted. In particular, inferential comparative genomic copy number (relative vitality), which is the relative amplification rate of cells related to the copy of genomic compounds in the cell comparing to those of the pure culture standards, are determined.
priorityDate 2015-06-15-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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