Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_1940f2ee244c81abf26027f91ee18e80 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_a017c72ae9ecedd4b85506c42208c8ff http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_660622f2aa1fa0dcaf8aac10ea28a41d http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_95c680d0d752da9f5e1f68078859cbe3 |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-7051 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B03D2203-003 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0636 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-56972 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5432 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-56966 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B03D1-1418 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01D21-262 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-54333 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B03D1-016 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/B01D21-26 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-569 |
filingDate |
2019-08-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_845325cc2450c3c5a58195c9cb4132e3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7842556f18fcf0f5abd80019326694ba http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_be3955fc55e349c7a2c21ad28996300f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7d2aea63f7edb79cd91f9fcb9bf6c54e |
publicationDate |
2020-03-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
US-2020072834-A1 |
titleOfInvention |
Methods for isolating target cells from blood |
abstract |
Disclosed herein are methods for isolating target cells from blood, involving mixing in an open container an undiluted blood sample having a volume of 10 ml or less, and binding agents, wherein each binding agent comprises (A) a primary binding agent comprising an agent capable of binding to at least one cellular epitope on target cells in the undiluted blood sample, (B) a first linker bound to the primary binding agent, to generate binding agent-attached target cells in the undiluted blood sample; contacting the binding agent-attached target cells in the undiluted blood sample with a plurality of buoyant reagents that include a second linker capable of binding to the first linker to generate an undiluted buoyant reagent-attached target cell mixture; diluting the undiluted buoyant reagent-attached target cell mixture by at least 20% to produce a diluted buoyant reagent-attached target cell mixture; applying a vectorial force, such as centrifugal force, to the diluted buoyant reagent-attached target cell mixture to generate a stratified diluted buoyant reagent-attached target cell mixture; removing the buoyant reagent-attached target cells from the stratified diluted buoyant reagent-attached target cell mixture; and isolating the target cells from the buoyant reagent-attached target cells. |
isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11583893-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11291931-B2 |
priorityDate |
2018-08-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |