| abstract |
Fluorescence resonance energy transfer (FRET) constructs comprising donor and acceptor fluorophore moieties, and a peptide linking the two, which is a substrate of the endopeptidase fibroblast activation protein (FAP). Also provided are isolated nucleic acids expressing the construct, cell lines comprising the nucleic acids, and kits comprising the construct. Further provided are methods of detecting FAP using the construct via FRET. |