| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_309fc743fd4529299614599357b75a58
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_3284babd59f13e5f8d0012df54dde5d4
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_e24952fca1fe54aebfc487740f7b67b1
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_a4aaf3eeafa7b29ee3e809ebf099b56c |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6853
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-689
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6893
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6895
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G16B20-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6809
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6837
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G16B30-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G16B25-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-686 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6893
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6895
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-689
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G16B30-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6806
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6809
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-686
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6837
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6853 |
| filingDate |
2019-09-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_dd2ea01a1b71135888d3f44e1c84df58
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1df9d089cbb03231c3dbc3095f93a500
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a446cd45334a28f61560fe14e3a92111 |
| publicationDate |
2020-01-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-2020017924-A1 |
| titleOfInvention |
Microarray Based Multiplex Pathogen Analysis and Uses Thereof |
| abstract |
Provided herein is a dual amplification method for identifying plant pathogens by analysis of pathogen DNA in an unpurified nucleic acid sample from the plant. Pathogen-specific and/or plant-specific primers are used to generate a first set of amplicons that are further amplified in a second amplification step using fluorescent tagged pathogen-specific primers. Fluorescent amplicons thus generated are hybridized with pathogen-specific nucleic acid probes that are immobilized on a solid support using bifunctional polymer linkers. The hybridized microarray is imaged to obtain fluorescent images of the amplicons and the nucleic acid probes, which are superimposed to detect the pathogen present in the plant. Also described herein is a method to identify a plant by analysis of plant DNA and a method to simultaneously detect both plant DNA and pathogen DNA in a single assay. |
| priorityDate |
2015-12-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |