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filingDate 2019-07-22-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7098a70443b0acff84bbd938e22a7fc9
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publicationDate 2020-01-09-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber US-2020010864-A1
titleOfInvention Recombinant Engineered Bacterium Co-Expressing Trans-Anethole Oxygenase and Formate Dehydrogenase and Application thereof in Production of Piperonal
abstract The present application discloses a method for producing piperonal by using a recombinant engineered bacterium co-expressing trans-anethole oxygenase and formate dehydrogenase, and an engineered bacterium thereof, including constructing a formate dehydrogenase gene fdh and trans-anethole oxygenase gene tao or trans-anethole oxygenase mutant gene co-expression recombinant vector; inductively expressing recombinant genetically engineered bacterium; and producing piperonal by using the recombinant genetically engineered bacterium. 15.91 g/L of piperonal with a transformation rate of 79.55% and a time-space transformation rate of 2.27 g/L/h can be finally obtained during catalysis, and the yield is significantly improved compared with the existing piperonal, thereby being more conducive to the smooth realization of industrial production.
isCitedBy http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111826332-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110408604-A
priorityDate 2018-07-04-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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