Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_cb351a5a4e03ee3773bd0df5dbae9ac3 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0071 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0077 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y101-99033 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P17-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0008 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0006 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y102-01002 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P17-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-02 |
filingDate |
2019-07-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7098a70443b0acff84bbd938e22a7fc9 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_75565432d464d28a22e98785c6175ef6 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_475642be9f51511546e10eaa9b13fc45 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_83d19c3292842b394ddfec2b30738ab8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c59aa5535ba8db56f3b843f4f5682490 |
publicationDate |
2020-01-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
US-2020010864-A1 |
titleOfInvention |
Recombinant Engineered Bacterium Co-Expressing Trans-Anethole Oxygenase and Formate Dehydrogenase and Application thereof in Production of Piperonal |
abstract |
The present application discloses a method for producing piperonal by using a recombinant engineered bacterium co-expressing trans-anethole oxygenase and formate dehydrogenase, and an engineered bacterium thereof, including constructing a formate dehydrogenase gene fdh and trans-anethole oxygenase gene tao or trans-anethole oxygenase mutant gene co-expression recombinant vector; inductively expressing recombinant genetically engineered bacterium; and producing piperonal by using the recombinant genetically engineered bacterium. 15.91 g/L of piperonal with a transformation rate of 79.55% and a time-space transformation rate of 2.27 g/L/h can be finally obtained during catalysis, and the yield is significantly improved compared with the existing piperonal, thereby being more conducive to the smooth realization of industrial production. |
isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111826332-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110408604-A |
priorityDate |
2018-07-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |