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filingDate 2019-07-03-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c9383a00f80c04a303651238b821dafb
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publicationDate 2020-01-09-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber US-2020010862-A1
titleOfInvention Long-chain dibasic acid with low content of fatty acid impurity and a method of producing the same
abstract The invention relates to a long-chain dibasic acid with low content of fatty acid impurity and a method of producing it, in particular to the preparation of a long-chain dibasic acid producing strain by using directed evolution and homologous recombination, and to the fermentation production of a long-chain dibasic acid with low content of fatty acid impurity by using said strain. The invention relates to an isolated mutated CPR-b gene, homologous gene or variant thereof, relative to the GenBank Accession Number AY823228, taking the first base upstream of the start codon ATG as −1, comprising a base mutation −322G>A, and taking the first base downstream of the stop codon TAG as 1, comprising mutations 3TUTR.19C>T and 3′UTR.76_77insT. The invention also relates to a strain containing said mutated CPR-b gene, homologous gene or variant thereof, wherein, when the strain is used to produce a long-chain dibasic acid by fermentation, the content of fatty acid impurity in the fermentation product is significantly decreased.
priorityDate 2018-07-06-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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