Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_2a819eda0adf22936a52362eeebb9fb4 |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K48-00 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K31-7088 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P35-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K41-0042 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K9-5169 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K9-5192 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K9-5138 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K38-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K47-58 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K31-7088 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K38-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K41-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K9-51 |
filingDate |
2019-08-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fa2278c13f2d297aacc7666438c42575 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_54a9222e2aec5e1908e03b5043f25b4f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_69b8ea4c98f0e96d1b8097630173d1f1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a5b452e240ba1b04b0a31ddecb81a22f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c66d6c355232211e3f8b0c13f4a7125d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_91e93b58f29b9a3d5ced1a95ba226a81 |
publicationDate |
2020-01-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
US-2020009070-A1 |
titleOfInvention |
Protease assisted native-protein delivery approach (panda) |
abstract |
A method for intracellular delivery of single proteins or other cargo molecules by encapsulation within nanocapsules formed by interfacial polymerization of one or more types of monomers and selected protease cleavable cross-linkers is provided. The thin positively charged capsules are readily brought into the cytosol of target cells by endocytosis. The capsules are degraded by the action of endogenous proteases or co-delivered proteases on the cross-linkers releasing the functional cargo unaltered. The cross-linkers can be adapted to be cleavable by specific enzymes selected from available intracellular enzymes within the target cell or co-delivered or self-cleaving when the cargo itself is a protease. The nanocapsules produced by the methods have been shown to have long-term stability, high cell penetration capability, low toxicity and efficient protease-modulated specific degradability without affecting cargo protein function. |
priorityDate |
2010-04-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |