| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_8073450a491d200d05b09b9beb6f0ec6
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_8e071d18fea054bf8d1420d483e78ed1 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-16 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1086
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1093
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1075
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6874
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6876
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6876
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6874 |
| filingDate |
2019-05-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_eb6a9416de73b5a8c0ea563bbd98b86c
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_63efacbafdafba105d4f74a855af88a2
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b6ba1ddded1bea15101295aa3369fa87
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1d6ed18febbf39b2bbdf168f5a398fba
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e59203914a1b205ef74ff6ad373bc324 |
| publicationDate |
2019-11-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-2019360044-A1 |
| titleOfInvention |
Multimodal readouts for quantifying and sequencing nucleic acids in single cells |
| abstract |
Provided herein are methods for generating single-cell molecular analysis comprising a) delivering one or more proximity dependent probes to a cell population, wherein each proximity dependent probe comprises a target binding region configured to bind a target RNA and a primer binding site region; b) linking bound proximity dependent probes; c) isolating single cells from the cell population in separate individual discrete volumes, the individual discrete volumes further comprising a primer pair and amplification reagents, wherein the primer pair binds to the primer binding sites of the ligation dependent probes, and wherein at least one primer comprises a barcode sequence that uniquely identifies the individual discrete volume; d) amplifying the ligated probes using the primer pair, wherein the barcode is incorporated into each resulting amplicon; and e) quantifying target RNAs in each individual cell based at least in part on sequencing the resulting amplicons. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2022192191-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/IT-201900024159-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2022197801-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11339427-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11761039-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2023139272-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2021146534-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2021252375-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2023148232-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2023030479-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2023004019-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2021119402-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2021128052-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2021124166-A1 |
| priorityDate |
2018-05-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |