Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_b79ea1c6c25451ae6f7c31cb9e750417 |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C40B50-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1096 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1068 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 |
filingDate |
2016-12-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c8ffbc8b6dc7e280d6b5559d40e3e3cc http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2d6fdc0f0447caf16cbd299030841f95 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9db60a9baecd7f6d9484cf9ae35f002f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_31e99a5dbd17841bbc29a2b0943ce01f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e6115fa21b28c58f5a6388c12b17804f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f830e1593190a01811a98e83ff4d6a6f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5c489294fa487f4c72765582535009d7 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_52d0f83e2b9b2789dfa4cca6eab68ef6 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_10718b620a158405e9e62bba45a373e6 |
publicationDate |
2019-11-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
US-2019338279-A1 |
titleOfInvention |
Method for constructing single cell sequencing library and use thereof |
abstract |
Provided in the present invention is a method for constructing a single cell sequencing library, comprising the following steps: a) lysing a single cell to obtain a single cell lysate; b) separating the nucleus and the cytoplasm in the single cell lysate obtained in step a) to obtain a nuclear solution and a total RNA solution; and c) constructing a chromatin DNA library with the nuclear solution obtained in step b) to obtain a chromatin-accessibility sequencing library of the single cell; and constructing a transcriptome library with the total RNA solution obtained in step b) to obtain a transcriptome sequencing library of the single cell. |
isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111575348-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112011596-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112280828-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2021226917-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114085897-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113462748-A |
priorityDate |
2016-12-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |