| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_a45ce70a0c36048e631b12c5b88f0e7f |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-92
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12R2001-125 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-75
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-50
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12R1-125
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N1-205
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-503 |
| classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-125 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-50 |
| filingDate |
2019-05-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c9dcd6e69b6b9bb0c620a1ef0c8dd8b8
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5613981c8571eba441135d0c9a49f7a6
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2a6e2bca082059065980011a37b91529
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_00fd5f730f00605e05a13be265b1fbae |
| publicationDate |
2019-11-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-2019338007-A1 |
| titleOfInvention |
Methods and means for expression of authentic and bioactive basic fibroblast growth factor in bacillus subtilis |
| abstract |
The present invention is concerned with a method of production of authentic and bioactive human basic fibroblast growth factor (bFGF) of 146 amino acids and without any modification at either C- or N-terminal of the bFGF. The method includes the steps as of providing a Bacillus subtilis host, introducing a DNA construct into the Bacillus subtilis host to produce a transformed Bacillus subtilis host, the DNA construct including an insert consisting of, from 5′ to 3′, a cellulose binding domain (CellBD), an intein sequence and a DNA coding for the bFGF polypeptide, and subjecting the transformed Bacillus subtilis host to a shake flask cultivation process or a fed-batch fermentation process. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-115976092-A |
| priorityDate |
2018-05-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |