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filingDate 2019-04-18-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2964b2dae486095ec2b6444fd19baf05
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publicationDate 2019-10-31-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber US-2019330598-A1
titleOfInvention Method for promoting replication of bovine rhinotracheitis viruses using cold atmospheric plasma
abstract A method for promoting replication of infectious bovine rhinotracheitis viruses using cold atmospheric plasma, including: irradiating a medium for Madin-Darby bovine kidney cells with a cold atmospheric plasma generator; adding the irradiated medium to the Madin-Darby bovine kidney cells; and adding infectious bovine rhinotracheitis viruses for incubation. The time required for treatment is 2 min allowing for a simple and rapid operation. The plasma is used for the indirect treatment of cells with a uniform process and a controllable intensity. The replication of the infectious bovine rhinotracheitis viruses in the Madin-Darby bovine kidney cells is significantly promoted by co-incubation in the treated DMEM for 1 hour, so that the high levels of infectious bovine rhinotracheitis viruses obtained can be used for vaccine production after inactivation, improving the vaccine production efficiency.
priorityDate 2018-04-25-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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