Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_cb351a5a4e03ee3773bd0df5dbae9ac3 |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-35 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2430-00 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07D211-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-9406 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-44 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K19-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-1289 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-795 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-577 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0693 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-163 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-065 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-44 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-12 |
filingDate |
2018-12-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b878bd2f7a50defa81edb32021216782 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f615d25bada2f40775e77645eb209857 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_aec56919af8c0bba0c9965baf53411d7 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_82b55446ab445087b783558d01227d1d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ffe6a734d4a81a9b6d2c830554a16747 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_87c6180cc5fd8e73ed36a294a868ecb0 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_467896d189dfa9ecaf01528986e8006a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_58dbbdf8ee4fef1f476418fba30ef704 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2f650cffbc34727d85b2abc338b73e2d |
publicationDate |
2019-09-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
US-2019284303-A1 |
titleOfInvention |
Hybridoma cell line of secreting cyproheptadine monoclonal antibodies and preparation method thereof |
abstract |
A hybridoma cell line of secreting cyproheptadine monoclonal antibodies with a preservation number of CGMCC No. 14699 belongs to the field of food safety immunological detection. BALB/c mice are immunized through one time immunization with complete freund's adjuvant, three times of booster immunization with incomplete freund's adjuvant and one time of rush immunization with cyproheptadine complete antigen without adjuvant; the spleen cells from BALB/C mice immunized with high potency and low value of IC50 are fused with murine myeloma cells; and then the hybridoma cell line is obtained through indirect competitive ELISA screening and three sub-clones. The monoclonal antibody secreted by this cell line has good specificity and detection sensitivity to cyproheptadine (value of IC50 is 0.37 ng/ml), being suitable for detection of cyproheptadine in food. |
isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113774030-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-116106536-A |
priorityDate |
2018-03-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |