| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_07d5f4667523063bce7464a42e3eb230 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-16
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-166
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-156 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6851
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6881 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6881
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6851
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6806 |
| filingDate |
2019-01-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c01bcd6838e5f4702e70666960612559
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5949ad27e5efaa821523879a8810c1ba
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_84ddb6595b6726c81da0a7399354d3dc
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c7a5b4c1283b0f4f88fcb2fc34b5e14d
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b9d6d6b47a0897e09760e64757fcb8f5 |
| publicationDate |
2019-07-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-2019203281-A1 |
| titleOfInvention |
Compositions and methods for measuring and calibrating amplification bias in multiplexed pcr reactions |
| abstract |
Compositions and methods are described for standardizing the DNA amplification efficiencies of a highly heterogeneous set of oligonucleotide primers as may typically be used to amplify a heterogeneous set of DNA templates that contains rearranged lymphoid cell DNA encoding T cell receptors (TCR) or immunoglobulins (IG). The presently disclosed embodiments are useful to overcome undesirable bias in the utilization of a subset of amplification primers, which leads to imprecision in multiplexed high throughput sequencing of amplification products to quantify unique TCR or Ig encoding genomes in a sample. Provided is a template composition comprising a diverse plurality of template oligonucleotides in substantially equimolar amounts, for use as a calibration standard for amplification primer sets. Also provided are methods for identifying and correcting biased primer efficiency during amplification. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11365411-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10526650-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2021150426-A1 |
| priorityDate |
2012-05-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |