| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_e5c34d1f925786388cf75cbf7922ca91 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C40B60-14
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-922 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6874
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L9-523
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-54313
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-54353 |
| classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C40B60-14 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/B01L9-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-543
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6874
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6869 |
| filingDate |
2018-06-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_17f90ba64e1d6f236ae2157033e3b096
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e4ffc4f8698aade7597906484662d265
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d415230c0a8204f04f3234b4e72eeb9d
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1475a35dfb2365b37784d420cd0ece44
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bb71ca21ccf7441e96c3ce65f57595bf
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3452faaa4d2b067fe90e82466bbfda98 |
| publicationDate |
2019-05-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-2019136313-A1 |
| titleOfInvention |
Loading extended polymerase-nucleic acid complexes |
| abstract |
Compositions, methods and systems are provided for the loading of extended polymerase-nucleic acid complexes onto substrates. Primed polymerase-template complex comprising a polymerase enzyme and a circular nucleic acid template comprising a double stranded region connected at each end by a single-stranded hairpin region are provides in which the circular nucleic acid template comprises at least one reversible pause point. Nucleic acid synthesis is carried out such that a nascent strand is synthesized up to the reversible stop point producing an extended polymerase-template complex. The extended polymerase-template complex is then attached to a substrate. Such attached complexes can be used for single molecule sequencing in which the nucleic acid synthesis is re-initiated such that nucleic acid synthesis continues past the reversible stop point. |
| priorityDate |
2011-03-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |