| abstract |
In one aspect, the present disclosure relates to a substantially albumin-free, chemically defined medium for efficiently supporting stem cell differentiation with significantly improved reproducibility and long-term culture of the differentiated cells. In particular embodiments, provided herein are compositions and methods for promoting atrial and ventricular cardiomyocytes formation from stem cells. The present disclosure further relates to the atrial and ventricular cardiomyocytes formed from the stem cells, and the uses of the cardiomyocytes, e.g., for cardiac injury repairmen, cardiac safety evaluation during drug discovery, and screening for new therapeutics for treating cardiac injuries. |