| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_bc2b03342511abc88f60c63f2acd96d0 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2310-20
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2310-313
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2310-311
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2310-3125
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2750-14143 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0665
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0635
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0636
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0647
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-85
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-907
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-113
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8645
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K48-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-805
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K35-28
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K31-7115
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P7-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-22 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K31-7115
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P7-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-0781
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-0783
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-0775
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-90
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-864
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-22
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-113 |
| filingDate |
2018-03-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d8f5585aa3dba6017670fe08eee26fc7
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a61f113e92909cfbc6b365c20945775f
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_129ec450f4291197f49fb4e60171efaa
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9a37f4dc2cc4630f13c5f214aec9ebfc
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_66d691b10edb14bf8087a59926813661 |
| publicationDate |
2019-01-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-2019032091-A1 |
| titleOfInvention |
Nuclease-Mediated Genome Editing of Primary Cells and Enrichment Thereof |
| abstract |
In certain aspects, the present invention provides methods for inducing a stable gene modification of a target nucleic acid via homologous recombination in a primary cell, such as a primary blood cell and/or a primary mesenchymal cell. In certain other aspects, the present invention provides methods for enriching a population of genetically modified primary cells having targeted integration at a target nucleic acid. The methods of the present invention rely on the introduction of a DNA nuclease such as a Cas polypeptide and a homologous donor adeno-associated viral (AAV) vector into the primary cell to mediate targeted integration of the target nucleic acid. Also provided herein are methods for preventing or treating a disease in a subject in need thereof by administering to the subject any of the genetically modified primary cells or pharmaceutical compositions described herein to prevent the disease or ameliorate one or more symptoms of the disease. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11634732-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2020227637-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11098325-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2022221319-A3
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11492646-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2023129947-A3
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2021211972-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114026243-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2021108665-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114945377-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11193141-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2021263179-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2020205604-A1 |
| priorityDate |
2015-09-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |