| abstract |
This invention relates to a method for obtaining immobilized enzyme preparations, in particular to preparation and application of noval active conjugate of enzyme with a polymer carrier. Said conjugate possesses the properties of Longidaza drug and inhibits hyperplasia of connective tissue, and anti-inflammatory action, as well as it can be used for manufacturing stable, active and safe in use long-acting drugs in the form of a suppository, ointment, injection or cosmetic cream, and for making veterinary drugs. This method consists in conjugation of hyaluronidase with a water-soluble copolymer using carbodiimide or azide method. The conjugation is carried out with the use of copolymer N-oxide 1,4-ethylene piperazine, (N-carboxymethyl)-1,4-ethylene piperazine or its hydrazide, and 1,4-ethylene piperazine of general formula n n n n n n n n n n where n is ranging from 40% to 90% of total number of cells; n m is ranging from 3% to 40% of total number of cells; n n+m+1=100%, |