| abstract |
The present disclosure discloses a primer combination and GeXP detection method for simultaneously identifying eight kinds of bovine pathogens. The primer combination of the present disclosure consists of primer pair I, primer pair II, primer pair III, primer pair IV, primer pair, primer pair VI, primer pair VII and primer pair VIII. The present disclosure also discloses a GeXP detection method that can simultaneously identify bovine infectious diseases of foot-and-mouth disease virus, bluetongue virus, vesicular stomatitis virus, bovine viral diarrhea virus, bovine rotavirus, enterotoxigenic E. coli, infectious bovine rhinotracheitis virus and peste des petits ruminants virus. The GeXP detection method established can simultaneously identify the eight pathogens of bovine infectious diseases. The method has the characteristics of high throughput, high specificity and sensitivity, and can be used for the bovine epidemiological monitoring and the differential diagnosis of sudden epidemic situation, and guarantees the healthy development of cattle industry. |