| abstract |
The present invention discloses a high volume manufacturing process for the production and purification of exosomes. In one embodiment, a hollow fiber perfusion reactor is used to expand stem cells. The growth media used from the stem cell expansion is captured, filtered, centrifuged and exosomes isolated from the waste effluent. In one embodiment, stem cells are derived from bone marrow, fat, blood, cord blood and induced pluripotent stem cells are expanded and the exosomes captured from the growth waste effluent. The exosomes can be used as therapeutics and diagnostics for a number of regenerative and chronic diseases. |