Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_cb351a5a4e03ee3773bd0df5dbae9ac3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_ddc350988c432a8e4c18f6cb124fa3e5 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_1288ef78363373c44950543d1524efe9 |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-61 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-815 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-24 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-62 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-81 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-50 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y301-01004 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-38 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-62 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-50 |
filingDate |
2018-06-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7c6d502bf57b68e2375d6955e0f0deca http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3f82de7ba3bf34eafadd1efd4c283901 |
publicationDate |
2018-09-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
US-2018265876-A1 |
titleOfInvention |
Methods for Improving Expression Levels of Foreign Proteins by Means of Phospholipase Fusion Expression |
abstract |
The invention discloses a method for improving the extracellular expression level of a foreign protein by means of phospholipase fusion expression. Four proteins, PLA 2 , MBP, CBD and SUMO, are used as a fusion tag to construct a fusion gene. Compared with an original protein MOH without any fusion tag, the extracellular expression level and enzymatic activity of all the four fusion proteins are increased to some degree. Among them, the fusion protein using PLA 2 as the fusion tag has the highest expression level, which is 7.4 times higher than that of the original protein. Compared with other fusion tags, PLA 2 has a low molecular weight and the fusion protein having PLA2 as the fusion tag has the highest expression level (up to 12 g·L −1 in a 7 L fermentation tank for high-density fermentation). It is shown that the secretory expression of a foreign protein can be effectively increased by using PLA 2 as a fusion tag. |
isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2716087-C1 |
priorityDate |
2016-01-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |