| abstract |
The invention relates to an immunoadsorbent for purifying five kinds of mycotoxins including fumonisin B 1 and aflatoxin B 1 , and a complex affinity column. The immunoadsorbent comprises a solid-phase support, and an anti-fumonisin B 1 monoclonal antibody, an anti-aflatoxin B 1 monoclonal antibody, an anti-ochratoxin A monoclonal antibody, an anti-zearalenone monoclonal antibody and an anti-sterigmatocystin monoclonal antibody which are coupled to the solid-phase support, wherein the anti-fumonisin B 1 monoclonal antibody is secreted by a hybridoma cell strain Fm7A11, and the hybridoma cell strain Fm7A11 has been preserved at China Center for Type Culture Collection, Wuhan University, Wuhan, China on Mar. 29, 2016 with the preservation number of CCTCC No. C201636. The complex affinity column can be used for purification and detection of a fumonisin B 1 sample, an aflatoxin B 1 sample, an ochratoxin A sample, a zearalenone sample and a sterigmatocystin sample at the same time. |