Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_b5095f60dee8e6d7c3faa7cca7b58c08 |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-2315 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-2321 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-2302 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2510-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2502-1107 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0646 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K35-17 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-0783 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K35-17 |
filingDate |
2016-08-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b5b81a4f3b67668dcffa9f5297c645fa http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_eea0a7f6c09b73a5950b51facbc2ca82 |
publicationDate |
2018-08-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
US-2018245044-A1 |
titleOfInvention |
Method for natural killer cell expansion |
abstract |
The present invention provides a method for in-vitro culturing and expanding natural killer (NK) cells in a cell culture medium comprising a population of NK cells, the method comprising a) adding an effective concentration of interleukin-21 (IL-21) at the beginning of the culturing process to said medium, b) adding repeatedly an effective concentration of interleukin-2 (IL-2) and/or interleukin-15 (IL-15) to said medium, and c) adding repeatedly feeder cells or membrane particles thereof to said medium, wherein said feeder cells are B cell derived which are EBV immortalized; and wherein said expansion of NK cells in said cell culture medium is maintained for at least 3 weeks. |
isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2021108389-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112680415-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112779161-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10590385-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11066644-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112553157-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112430573-A |
priorityDate |
2015-09-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |