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filingDate 2018-02-15-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 2018-08-16-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber US-2018230451-A1
titleOfInvention Systems and Processes for Rapid Nucleic Acid Extraction, Purification, and Analysis
abstract Disclosed are processes and kits for rapid nucleic acid extraction from a nucleic acid-containing material, such as a bone, tooth or semen sample. For bone and tooth process involves providing the nucleic acid-containing material in a form suitable for nucleic acid extraction, adding a lysis buffer to the nucleic acid-containing material to obtain a mixture, mixing the mixture in a manner equivalent for about 30 seconds or longer and separating the mixture by centrifugation to obtain a liquid supernatant. The liquid supernatant contains the extracted nucleic acids which can be used for analysis including STR profiling by conventional or rapid DNA analysis. For semen the processes and kits involve applying an appropriate amount of sperm disruptive agent.
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