| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_df809f80345d5a8e7cafd6fd1188a474 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1007
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2440-12
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2030-8831 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C40B20-08
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6812
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-14
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-96 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-14
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C40B20-08
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-68 |
| filingDate |
2016-06-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_11788d4c44eaf5a923857008235b06c1
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5d7afb0d4ccf665bbf31514eddd2f439
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bc8fe5977ba6cb31256675d25ee24775 |
| publicationDate |
2018-07-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-2018188262-A1 |
| titleOfInvention |
Method for profiling protein methylation |
| abstract |
There is provided a method for identifying protein methylation on arginine and lysine residues. The method comprises obtaining a set of peptides; blocking un-methylated arginine and lysine residues and the free N-terminal amine of peptides in the set of peptides, so that un-methylated peptides are neutralized and only methylated peptides are positively charged at neutral or basic pH; isolating the methylated peptides based on charge; and performing mass spectrometry (MS) analysis on the isolated methylated peptides to detect methylated lysine and arginine residues. Methods provided herein can be used for large scale, high throughput profiling of protein methylation in a cell or tissue. |
| priorityDate |
2015-06-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |