| abstract |
Provided is a cell line deposited under the number of CGMCC11091 and preparation method thereof. The method comprises: using a critical downstream gene of the BMP signal, the Id1 gene, as a target, then designing dual reporter gene elements to insert in an Id1 genome, so as to construct a recombination vector, and transcribing the same in a human embryonic stem cell line. A novel compound upregulating expression of Id1 and obtained by screening with the cell line has an application in preparing a pharmaceutical treating pulmonary arterial hypertension. |