http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2018113114-A1
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_134e761e8dcbff4985fa1e77a9c2062e |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5005 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-502 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5073 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-50 |
filingDate | 2017-08-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_04b1a016e2a254fbcc4c501b2b93d2f0 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7c80abc2ad8e5df0b83f42bfedd2fa34 |
publicationDate | 2018-04-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | US-2018113114-A1 |
titleOfInvention | Arrays of discrete cell culture microenvironments, methods of making such arrays and uses thereof |
abstract | A method of making an array having discrete volumes of cell culture micro-environments possessing different properties influencing the behavior of encapsulated cells, in particular proliferation, colony-formation, differentiation, migration, or combinations thereof. The method comprises a) providing different hydrogel precursor molecules, where the precursor molecules are linear or branched hydrophilic polymers for building up cell culture micro-environments; b) combining and dispensing different combinations of the hydrogel precursor molecules, according to step a), onto discrete volumes of a multi-well plate; c) adding one or more biologically active molecules to the discrete volumes and either attaching the molecules to one of the hydrogel precursor molecules present or a hydrogel, formed in step e), or allowing them to diffuse freely; d) adding cells onto/into the discrete volumes of the multi-well plate; and e) crosslinking the hydrogel precursor molecules based on an enzymatically catalyzed reaction or a Michael addition reaction to form a hydrogel matrix. |
priorityDate | 2013-05-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 1138.