Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_e00480388d1e32d691e588287ae55e71 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_eadaa85202d61dd5e4cad1c3fae02980 |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-80 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-107 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-90 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-60 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2830-205 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-63 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-79 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-635 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-85 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-63 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-85 |
filingDate |
2016-03-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1f1a24c1e60b70fb107dc55cdbfc4d35 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1dc5ddda0601bbad5c134bdd18249968 |
publicationDate |
2018-04-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
US-2018105833-A1 |
titleOfInvention |
System for stable gene expression |
abstract |
An inducible expression cassette for controlled expression of multiple genes includes the DNA sequences of a bidirectional promoter inserted between two DNA sequences. Transfection into a host cell and the two DNA sequences encode genes of interest, provides one DNA sequence expressed constitutively and manipulation of the expression of the other DNA sequence. A regulatory DNA cassette containing another bidirectional promoter and two DNA sequences that encode a marker and a regulatory expression product is also disclosed. Both cassettes can be incorporated in a non-viral vector, like the Sleeping Beauty transposon, or a viral vector to induce controlled expression of multiple genes into host cells. A kit containing a package of each above vector type is also disclosed, as is a method of transforming a host cell. |
isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2022266083-A3 |
priorityDate |
2015-03-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |