| abstract |
What is described is a trinucleotide cap analog comprising m 7 G(5′)p 3 -N 1 pN 2 for increased efficiency of in vitro transcription of m7G(5′)p 3 -RNA, wherein m 7 G is N 7 -methylguanosine or analog, (5′)p 3 is a 5′,5′-triphosphate bridge, and N 1 or N 2 or both ribonucleotide analogs linked to each other by a phosphate, p, and wherein the trinucleotide cap analog increases the efficiency of in vitro transcription. |