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publicationDate 2018-04-19-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber US-2018103845-A1
titleOfInvention Method for assessing irritancy to biological samples
abstract A method for assessing irritancy to a biological sample of the present invention is a method for assessing irritancy of a chemical to a three-dimensional biological sample having a plurality of layers in the depth direction and includes: image acquisition step of acquiring, at different depths of the biological sample, fluorescence images of a nuclei of cells in at least one group of a living cell group and a dead cell group in the biological sample exposed to the chemical, the nuclei of cells in at least one group being labeled with a fluorescent marker; and assessment step of calculating the ratio of the number of cells in the at least one cell group existing in the depth direction on the basis of the plurality of fluorescence images.
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