http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2018067123-A1
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_a05de5345a1d16e34603ae07b8e52a9a |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6827 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6806 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-31 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-68 |
filingDate | 2017-08-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_60f0809fb7021a10870056860b621fe3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_70944b36ca7bbd4cae5164dc0972137e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_925bd3c5caead986bbecc329b0481231 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_62bf2f5a638c09f4dc90adf3d74f86d2 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5b01eb2d1da1209881fc1b2bfe6b7abf http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_976f66a5b2ea573de96c161e1bb11025 |
publicationDate | 2018-03-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | US-2018067123-A1 |
titleOfInvention | Protein assay method |
abstract | A protein assay method, including: 1) dissolving 4-hydroxybenzenesulfonic acid in an ethanol, methanol, or acetonitrile aqueous solution to prepare a hydrolysis reagent, adding a protein sample and the hydrolysis reagent to a hydrolysis tube, and uniformly mixing the protein sample and the hydrolysis reagent, charging argon or nitrogen into the hydrolysis tube to remove dissolved oxygen, sealing and drying the hydrolysis tube, and hydrolyzing the protein sample; 2) cooling the hydrolysis tube to room temperature, filtering and adding a resulting hydrolysate to a volumetric flask, washing the hydrolysis tube and a filter paper, collecting and adding a resulting washing solution to the volumetric flask to a constant volume; 3) drawing a hydrolysate solution from the volumetric flask, completely drying the hydrolysate solution to yield a solid product; and 4) diluting the solid product using a diluent, and analyzing amino acids of the solid product. |
priorityDate | 2016-09-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 57.