| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_2a819eda0adf22936a52362eeebb9fb4 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02E60-10 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-025
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61N1-303
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61N1-325
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12M35-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12M23-04
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/H01M4-66 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12M1-12
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12M1-42
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/H01M4-66 |
| filingDate |
2017-08-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bf1d579f182687e781c997ae69938f75
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_06683fda7d07cbf292dc44f927282244
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_15985074c2006deb12df988cde717806 |
| publicationDate |
2018-03-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-2018066222-A1 |
| titleOfInvention |
Device for massively parallel high throughput single cell electroporation and uses thereof |
| abstract |
In various embodiments a Massively parallel Single-cell Electroporation Platform (MSEP) for low voltage, high efficiency delivery of extracellular materials into mammalian cells at an ultrahigh throughput of 10 million cells/min on a 1 cm 2 chip is provided. In certain embodiments MSEP is realized by a 3D silicon-based device with, e.g., 5,000 short vertical microfluidic channels in parallel. Single cells flowing through these channels are geometrically confined to regions with intense and localized electric fields where cells are electroporated. High efficiency delivery of calcium dyes, large-sized dextran proteins, and plasmids into mammalian cells to establish a range of sizes and compositions have been successfully accomplished with MSEP. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2020393439-A1 |
| priorityDate |
2016-08-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |