| abstract |
In an aspect of the present invention, in order to (i) clear the biological material by reducing light scattering while preserving fine morphology of a biological material and preserving fluorescence of a fluorescent substance, and (ii) reduce a spherical aberration in observation under a microscope, the biological material is cleared by being immersed in a solution containing a detergent and a non-ionic organoiodine compound, and then the cleared biological material is observed under a microscope in a state of being immersed in a solution that contains a non-ionic organoiodine compound and that has a refractive index higher than 1.48. |