| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_0cec25d61998774f1f7cba37cade1635 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-66
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6874
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6855
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1093
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 |
| filingDate |
2017-07-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ad6184159965d9a9233ac5a018901dac
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3cb9cfa5f957e259e2c21b629b6854fe
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8ba95f62f91bc3f80d8e8257ecdc789e
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_83ca7af091df5c4a23cc75ca7ad94d72
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_262f11d89b18451762f0e4ce3aac9921
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ccb73d1a6270c01211cf883783629b1c |
| publicationDate |
2018-01-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-2018010178-A1 |
| titleOfInvention |
Coupling adaptors to a target nucleic acid |
| abstract |
Methods of coupling adaptors to a target nucleic acid include coupling a first adaptor to a first end of the target nucleic acid to form a coupled first adaptor. A portion of a second adaptor is hybridized to a portion of the coupled first adaptor to form a hybridized second adaptor having a single-stranded 3′-end. The hybridized second adaptor is coupled to a second end of the target nucleic acid to form an adaptor-flanked product having at least a part of the first adaptor coupled to the first end of the target nucleic acid and at least a part of the second adaptor coupled to the second end of the target nucleic acid. These methods can minimize the formation of adaptor-dimers that may be problematic in subsequent complementary nucleic acid strand synthesis, amplification, and sequencing. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109457299-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11390915-B2 |
| priorityDate |
2016-07-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |